Curation Information

Characterization and functional analysis of the poxB gene, which encodes pyruvate oxidase in Lactobacillus plantarum.;Lorquet F, Goffin P, Muscariello L, Baudry JB, Ladero V, Sacco M, Kleerebezem M, Hols P;Journal of bacteriology 2004 Jun; 186(12):3749-59 [15175288]
CcpA [F9UQH6, view regulon]
Reported TF sp.
Lactobacillus plantarum LM3
Reported site sp.
Lactobacillus plantarum LM3
Created by
Dinara Sagitova
Curation notes

Experimental Process

The transcription start site of the poxB gene was mapped by primer extension analysis. A putative CcpA binding site was identified by visual inspection of the poxB promoter. Primer extension experiments in the wild type and ccpA mutant strains identified that poxB transcription was repressed by CcpA. An in vivo footprinting analysis showed that there was a CcpA-dependent protection of the CcpA binding site in the poxB promoter sequence.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... pox5
Experimental technique details Premethylation interference footprinting (ECO:0005656) - Experimental technique details Primer Extension assay (ECO:0005657) - Experimental technique details Visual sequence inspection (nan) - repressor not specified