Visual inspection of the ily promoter identified a sequences similar to the CcpA consensus sequence. Site-directed mutagenesis was used to introduce point and deletion mutations into the putative CcpA binding site. qRT-PCR showed that partial derepression of ily occurred in the strains containing mutated CcpA promoters whereas ily was repressed in the wild type strain. EMSA demonstrated that binding was abolished in a promoter containing a mutated CcpA binding site whereas specific binding was observed when the wild type promoter sequence was used.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|