Curation Information

The Bacillus subtilis response regulator gene degU is positively regulated by CcpA and by catabolite-repressed synthesis of ClpC.;Ishii H, Tanaka T, Ogura M;Journal of bacteriology 2013 Jan; 195(2):193-201 [23123903]
CcpA [P25144, view regulon]
Reported TF sp.
Bacillus subtilis subsp. subtilis str. 168
Reported site sp.
Bacillus subtilis subsp. subtilis str. 168
Created by
Dinara Sagitova
Curation notes

Experimental Process

CcpA-mediated regulation was confirmed by degU-lacZ reporter assays. The induction of degU by glucose was abolished by disruption of ccpA. EMSA analysis confirmed direct binding of CcpA to the degU promoter region. DNase I footprinting confirmed that CcpA binds to cre sequence in the degU promoter. Site-directed mutagenesis of the CcpA binding site resulted in significant reduction in binding. The functionality of the CcpA binding sequence was confirmed in vivo by lacZ reporter assays in conjunction with site-directed mutagenesis.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... degU degS yviA comFA comFB comFC yvyF flgM yvyG flgK flgL yviE yviF csrA hag yvyE yvhJ
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - Experimental technique details Western blot (quantitative) expression analysis (ECO:0000279) - activator not specified