Curation Information

Mutational analysis of the bglH catabolite-responsive element (cre) in Lactobacillus plantarum.;Marasco R, Muscariello L, Rigano M, Sacco M;FEMS microbiology letters 2002 Feb 19; 208(1):143-6 [11934508]
CcpA [F9UQH6, view regulon]
Reported TF sp.
Lactobacillus plantarum LM3
Reported site sp.
Lactobacillus plantarum LM3
Created by
Dinara Sagitova
Curation notes

Experimental Process

EMSA confirmed binding of CcpA to the bglH promoter region. Introduction of a single substitution in the CcpA binding site drastically lowered the affinity of cre–protein binding in vitro. Comparison of the expression level of the wild type and mutated bglH promoter with the cat reporter gene confirmed CcpA-mediated repression of bglH.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... pbg9
Experimental technique details CAT reporter gene assays (ECO:0005617) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified