EMSA confirmed binding of CcpA to the bglH promoter region. Introduction of a single substitution in the CcpA binding site drastically lowered the affinity of cre–protein binding in vitro. Comparison of the expression level of the wild type and mutated bglH promoter with the cat reporter gene confirmed CcpA-mediated repression of bglH.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|