EMSA and site directed mutagenesis confirmed the sole binding site of GlxR in the ramA promoter that was identified previously by chromatin immunoprecipitation analysis. Site directed mutagenesis and lacZ analysis showed that GlxR activates ramA expression.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|