A putative CodY binding site on the ureI promoter was identified by comparison with the CodY consensus binding site. CAT reporter assay and urease assays were both used to demonstrate repression of the ureI promoter by CodY. ChIP-PCR of ureC mRNA demonstrated lower transcription rates in the wild type than in the CodY mutant strain, confirming negative regulation. Data also demonstrated CodY to be modulated by growth pH, and suggested ureI is regulated by additional transcription factors. EMSA confirmed specific binding of CodY to the ureI promoter.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|AAATTTCTGAAAATT||ureI, ureA, ureB, ureC, ureE, ureF, ureG, ureD, ureM, ureQ, ureO,||