Curation Information

The pH-dependent expression of the urease operon in Streptococcus salivarius is mediated by CodY.;Huang SC, Burne RA, Chen YY;Applied and environmental microbiology 2014 Jun 20; (): [24951785]
CodY [A0A074IWI7, view regulon]
Reported TF sp.
Streptococcus salivarius 57.I
Reported site sp.
Streptococcus salivarius 57.I
Created by
Matthew Coveyou
Curation notes

Experimental Process

A putative CodY binding site on the ureI promoter was identified by comparison with the CodY consensus binding site. CAT reporter assay and urease assays were both used to demonstrate repression of the ureI promoter by CodY. ChIP-PCR of ureC mRNA demonstrated lower transcription rates in the wild type than in the CodY mutant strain, confirming negative regulation. Data also demonstrated CodY to be modulated by growth pH, and suggested ureI is regulated by additional transcription factors. EMSA confirmed specific binding of CodY to the ureI promoter.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
AAATTTCTGAAAATT ureI, ureA, ureB, ureC, ureE, ureF, ureG, ureD, ureM, ureQ, ureO,
... ... ureI ureA ureB ureC ureE ureF ureG ureD ureM ureQ ureO Ssal_01904
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details CAT reporter gene assays (ECO:0005617) - Experimental technique details ChIP-PCR (ECO:0005620) - Experimental technique details Consensus search (ECO:0005624) - Experimental technique details EMSA (ECO:0001807) - repressor not specified