EMSA showed that CRP bound to the tonB3 promoter region. In silico analysis identified a putative CRP binding site. DNase I footprinting showed that CRP protected region overlapped the predicted CRP consensus sequence. Beta-gal. reporter assays showed that the expression of tonB3 was significantly lower in the crp mutant than in the wild-type strain.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|