Curation Information

Low cell density regulator AphA upregulates the expression of Vibrio vulnificus iscR gene encoding the Fe-S cluster regulator IscR.;Lim JG, Park JH, Choi SH;Journal of microbiology (Seoul, Korea) 2014 May; 52(5):413-21 [24535746]
AphA [Q7MMB6, view regulon]
Reported TF sp.
Vibrio vulnificus MO6-24/O
Reported site sp.
Vibrio vulnificus MO6-24/O
Created by
Dinara Sagitova
Curation notes

Experimental Process

Primer extension analyses showed that PiscR activity was significantly increased in the iscR mutant and decreased in the aphA mutant. EMSAs showed that IscR and AphA bound specifically to the iscR promoter. DNase I footprinting analysis identified the AphA binding region in the iscR promoter. qRT-PCR showed that AphA activated the iscR expression only in the presence of functional IscR.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... VVMO6_02440 VVMO6_02439 VVMO6_02438 VVMO6_02437 VVMO6_02436 VVMO6_02435 VVMO6_02434 VVMO6_02433
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Primer Extension assay (ECO:0005657) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified