Primer extension analyses showed that PiscR activity was significantly increased in the iscR mutant and decreased in the aphA mutant. EMSAs showed that IscR and AphA bound specifically to the iscR promoter. DNase I footprinting analysis identified the AphA binding region in the iscR promoter. qRT-PCR showed that AphA activated the iscR expression only in the presence of functional IscR.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|