Curation Information

The Type II Secretion Pathway in Vibrio cholerae Is Characterized by Growth Phase-Dependent Expression of Exoprotein Genes and Is Positively Regulated by σE.;Zielke RA, Simmons RS, Park BR, Nonogaki M, Emerson S, Sikora AE;Infection and immunity 2014 Jul; 82(7):2788-2801 [24733097]
RpoE [Q9KPA6, view regulon]
Reported TF sp.
Vibrio cholerae O395
Reported site sp.
Vibrio cholerae O395
Created by
Dinara Sagitova
Curation notes

Experimental Process

A putative sigma-E binding site that had one mismatch with the V.cholerae and E.coli sigma-E consensus was identified in the eps regulatory region. rpoEP2-lux promoter fusion showed that s iron starvation and exposure to polymyxin B resulted in 4- and 6-fold increases, respectively, in transcriptional activity. esp-lux fusion with an esp promoter that lacked a putative sigma-E binding site showed that RpoE had no effect on the expression compared to the wild type promoter fusion in which overexpression of RpoE resulted in over 4-fold induction of the esp expression. The immunoblotting analyses showed that RpoE positively regulated the expression of T2S at the protein level. Peps-lux fusion assays in wild type O395 and the rpoE mutant strains showed that there was a statistically significant 2-fold induction of eps-lux activity in the wild-type V. cholerae.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... epsC gspD epsE epsF epsG epsH epsI epsJ epsK epsL epsM epsN hslO
Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details Visual sequence inspection (nan) - activator not specified