Curation Information

2-chloromuconate and ClcR-mediated activation of the clcABD operon: in vitro transcriptional and DNase I footprint analyses.;McFall SM, Parsek MR, Chakrabarty AM;Journal of bacteriology 1997 Jun; 179(11):3655-63 [9171413]
ClcR [A0A024HKB0, view regulon]
Reported TF sp.
Pseudomonas putida PRS2000
Reported site sp.
Pseudomonas putida PRS2000
Created by
Matthew Coveyou
Curation notes

Experimental Process

Beta-gal assay indicated an intact clcA gene was necessary for activation of the clcAB operon, suggesting clcA coded the enzyme responsible for synthesizing the clcR inducer. In-vivo transcription confirmed this. DNase I protection identified the putative clcR binding site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... clcA clcR catB2 bug36 Bpet1537 Bpet1538 Bpet1539
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - activator not specified