Beta-gal assay of both the clcA and clcR genes demonstrated activation and autoregulation by clcR, respectively. EMSA confirmed specific binding of clcR to the clcA-clcR promoter region. Comparison of the promoter region with a proposed LysR family consensus binding site identified a putative binding site for clcR.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|