brlR transcription start site was identified by 5'RACE. EMSA showed that BrlR specifically binds to its own promoter. ChIP-qPCR confirmed BrlR binding to its promoter in vivo. Site-directed mutagenesis of the putative BrlR binding site combined with EMSA showed that mutating one half of the palindromic sequence impaired BrlR binding. Promoter-lacZ fusion assays showed that brlR expression is enhanced by c-di-GMP. MEME motif discovery algorithm was used to identify the BrlR binding motif.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|