DNase I protection of the plasmid (pAC27)-borne clcABD promoter and chromosomal catBC promoter generated a binding footprint for CatR on each. CatR was shown to bind to clcABD without its inducer, though in the presence of the inducer binding affinity was increased. EMSA confirmed binding to both regions. Qualitative growth analysis and beta-gal assay together demonstrated catBC and clcABD activation by catR, with the later being activated even without the inducer.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|