EMSA, DNase I footprinting, and motif discovery determined three binding sites for GlxR: two in the genR-KH promoter region (R-KHx01, R-KHx02) and one in the genDMF promoter region (DMFx). Site directed mutagenesis and Beta-gal. assays determined that genDMF, genR, and genKH are repressed by GlxR.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|