EMSA and DNase I footprinting demonstrated that RpoN binds to the σ54-promoter consensus sequence within the algD promoter. Site-directed mutagenesis with xylE reported assays showed that a mutation in the RpoN binding site abolished the repression of algD by RpoN.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|