Curation Information

Dual regulation of mucoidy in Pseudomonas aeruginosa and sigma factor antagonism.;Boucher JC, Schurr MJ, Deretic V;Molecular microbiology 2000 Apr; 36(2):341-51 [10792721]
RpoN [P24255, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO381
Created by
Dinara Sagitova
Curation notes

Experimental Process

EMSA and DNase I footprinting demonstrated that RpoN binds to the σ54-promoter consensus sequence within the algD promoter. Site-directed mutagenesis with xylE reported assays showed that a mutation in the RpoN binding site abolished the repression of algD by RpoN.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... algD alg8 alg44 algK algE algG algX algL
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator not specified