Northern blot analysis and LacZ reporter assays demonstrated that BphS negatively regulates bph operon expression. bphE transcription start site was identified by primer extension analysis. BphS binding region was identified by deletion analysis of the bphE promoter with lacZ reporter assays. EMSA confirmed BphS binding to this region. BphS binding sites were localized by DNase I footprinting assays.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|