Curation Information

Critical nucleotides in the interaction of CatR with the pheBA promoter: conservation of the CatR-mediated regulation mechanisms between the pheBA and catBCA operons.;Tover A, Zernant J, Chugani SA, Chakrabarty AM, Kivisaar M;Microbiology (Reading, England) 2000 Jan; 146 ( Pt 1)():173-83 [10658664]
CatR [Q88GK5, view regulon]
Reported TF sp.
Pseudomonas putida PaW85
Reported site sp.
Pseudomonas putida plasmid pAT1140
Created by
Matthew Coveyou
Curation notes

Experimental Process

CatR regulation of the pheBA operon in Pseudomonas putida was investigated as it related to an internal binding site (IBS), identified by DNase I protection in an earlier publication. Deletion analysis and site-directed mutagenesis demonstrated reduced pheBA expression so long as both the recognition binding site (RBS) and activation binding site (ABS) were intact.

Transcription Factor Binding Sites

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type