In-vitro transcription assay demonstrated strong transcription of the clcABD operon only in the presence of both ClcR and its inducer (2-CM). DNase I protection revealed two binding regions, the recognition binding site (RBS) and activation binding site (ABS). The protection pattern of the ABS shifted when ClcR was influenced by its inducer.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|