Curation Information

DNase I footprinting, DNA bending and in vitro transcription analyses of ClcR and CatR interactions with the clcABD promoter: evidence of a conserved transcriptional activation mechanism.;McFall SM, Klem TJ, Fujita N, Ishihama A, Chakrabarty AM;Molecular microbiology 1997 Jun; 24(5):965-76 [9220004]
ClcR [A0A024HKB0, view regulon]
Reported TF sp.
Pseudomonas putida plasmid pAC27
Reported site sp.
Pseudomonas putida plasmid pAC27
Created by
Matthew Coveyou
Curation notes

Experimental Process

In-vitro transcription assay demonstrated strong transcription of the clcABD operon only in the presence of both ClcR and its inducer (2-CM). DNase I protection revealed two binding regions, the recognition binding site (RBS) and activation binding site (ABS). The protection pattern of the ABS shifted when ClcR was influenced by its inducer.

Transcription Factor Binding Sites

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type