Curation Information

DNase I footprinting, DNA bending and in vitro transcription analyses of ClcR and CatR interactions with the clcABD promoter: evidence of a conserved transcriptional activation mechanism.;McFall SM, Klem TJ, Fujita N, Ishihama A, Chakrabarty AM;Molecular microbiology 1997 Jun; 24(5):965-76 [9220004]
CatR [Q88GK5, view regulon]
Reported TF sp.
Pseudomonas putida PRS3026
Reported site sp.
Pseudomonas putida plasmid pAC27
Created by
Matthew Coveyou
Curation notes

Experimental Process

In-vitro transcription assay indicated clcABD activation by CatR even in the absence of its inducer (CCM), though activation is increased in the presence of CCM. Comparative DNase I protection assays between CatR and ClcR revealed the region protected by ClcR with its inducer (2-CM) is identical to that protected by CatR without CCM.

Transcription Factor Binding Sites

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type