Primer extension analysis using RNA from the wild type P. aeruginosa FRD1 and algT mutant strains determined that algZ was not expressed in algT mutant. Visual inspection of the algZ promoter identified a putative AlgT binding site with a partial match to AlgT consensus sequence. Site-directed mutagenesis of the putative binding site showed that compared to the wild type expression level, mutations in the AlgT binding site resulted in significant reduction in algZ transcription.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|