Curation Information

Control of Pseudomonas aeruginosa algZ expression by the alternative sigma factor AlgT.;Wozniak DJ, Sprinkle AB, Baynham PJ;Journal of bacteriology 2003 Dec; 185(24):7297-300 [14645293]
AlgU [Q06198, view regulon]
Reported TF sp.
Pseudomonas aeruginosa FRD1
Reported site sp.
Pseudomonas aeruginosa FRD1
Created by
Dinara Sagitova
Curation notes

Experimental Process

Primer extension analysis using RNA from the wild type P. aeruginosa FRD1 and algT mutant strains determined that algZ was not expressed in algT mutant. Visual inspection of the algZ promoter identified a putative AlgT binding site with a partial match to AlgT consensus sequence. Site-directed mutagenesis of the putative binding site showed that compared to the wild type expression level, mutations in the AlgT binding site resulted in significant reduction in algZ transcription.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... amrZ phnC PA3383 phnE PA3381 PA3380 PA3379 PA3378 PA3377 PA3376 PA3375 PA3374 PA3373 PA3372
Experimental technique details Beta-gal reporter assay - Experimental technique details Primer Extension assay (ECO:0005657) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator not specified