Transcriptional start sites for phzA and phzR were identified by primer extension analysis. A putative PhzR binding site was identified in the phzA-phzR intergenic region by visual inspection. Beta-gal reporter fusions confirmed that an intact phz box is required for PhzR-mediated activation of the phzA and phzR genes.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|