Curation Information

Activation of the phz operon of Pseudomonas fluorescens 2-79 requires the LuxR homolog PhzR, N-(3-OH-Hexanoyl)-L-homoserine lactone produced by the LuxI homolog PhzI, and a cis-acting phz box.;Khan SR, Mavrodi DV, Jog GJ, Suga H, Thomashow LS, Farrand SK;Journal of bacteriology 2005 Sep; 187(18):6517-27 [16159785]
PhzR [G3XD77, view regulon]
Reported TF sp.
Pseudomonas fluorescens 2-79
Reported site sp.
Pseudomonas fluorescens 2-79
Created by
Dinara Sagitova
Curation notes
P. fluorescens and P. aeruginosa genomes are not matching at the species level.

Experimental Process

Transcriptional start sites for phzA and phzR were identified by primer extension analysis. A putative PhzR binding site was identified in the phzA-phzR intergenic region by visual inspection. Beta-gal reporter fusions confirmed that an intact phz box is required for PhzR-mediated activation of the phzA and phzR genes.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... phzA1 phzM phzB1 phzC1 phzD1 phzE1 phzF1 phzG1
Experimental technique details Beta-gal reporter assay - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator not specified