Curation Information

Activation of the catBCA promoter: probing the interaction of CatR and RNA polymerase through in vitro transcription.;Chugani SA, Parsek MR, Hershberger CD, Murakami K, Ishihama A, Chakrabarty AM;Journal of bacteriology 1997 Apr; 179(7):2221-7 [9079907]
CatR [Q88GK5, view regulon]
Reported TF sp.
Pseudomonas putida PRS2000
Reported site sp.
Pseudomonas putida PRS2000
Created by
Matthew Coveyou
Curation notes

Experimental Process

In vitro transcription assay of the Pseudomonas putida catBCA promoter demonstrated dependence on both catR and the inducer molecule. DNase I protection in inducer-variable solution identified two catR binding sites, the recognition binding site (RBS) and activation binding site (ABS). The latter was confirmed by methylation interference assay. ABS was only bound in the presence of the inducer, while RBS was found to be inducer-independent.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... catB catC catA catR PP_3712
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details In-vitro transcription (ECO:0001204) - Experimental technique details Premethylation interference footprinting (ECO:0005656) - activator tetramer