Two CatR binding sites in the catR and catBC promoter region were identified by DNase I protection assay, a recognition binding site (RBS) and activation binding site (ABS), and significant nucleotides were identified by methylation interference footprinting. The ABS was protected only in the presence of the inducer, while RBS was bound constitutively. EMSA of RBS and ABS variable mutant strains in inducer-variable solution indicated cooperative binding; ABS required a functional RBS to bind, though RBS could be bound independent of the ABS
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|