Curation Information

Roles of CatR and cis,cis-muconate in activation of the catBC operon, which is involved in benzoate degradation in Pseudomonas putida.;Parsek MR, Shinabarger DL, Rothmel RK, Chakrabarty AM;Journal of bacteriology 1992 Dec; 174(23):7798-806 [1447146]
CatR [F8G324, view regulon]
Reported TF sp.
Pseudomonas putida PRS2000
Reported site sp.
Pseudomonas putida PRS2000
Created by
Matthew Coveyou
Curation notes
The RBS is described as an autorepression binding site for the catR gene based upon evidence from an earlier publication, but no supporting expression data is present in this publication.

Experimental Process

Two CatR binding sites in the catR and catBC promoter region were identified by DNase I protection assay, a recognition binding site (RBS) and activation binding site (ABS), and significant nucleotides were identified by methylation interference footprinting. The ABS was protected only in the presence of the inducer, while RBS was bound constitutively. EMSA of RBS and ABS variable mutant strains in inducer-variable solution indicated cooperative binding; ABS required a functional RBS to bind, though RBS could be bound independent of the ABS

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... PPS_3181 PPS_3180 PPS_3182 PPS_3179 PPS_3183
Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Premethylation interference footprinting (ECO:0005656) - activator tetramer