Curation Information

Functional analysis of the Pseudomonas putida regulatory protein CatR: transcriptional studies and determination of the CatR DNA-binding site by hydroxyl-radical footprinting.;Rothmel RK, Shinabarger DL, Parsek MR, Aldrich TL, Chakrabarty AM;Journal of bacteriology 1991 Aug; 173(15):4717-24 [1649820]
CatR [Q88GK5, view regulon]
Reported TF sp.
Pseudomonas putida PRS2000
Reported site sp.
Pseudomonas putida PRS2000
Created by
Matthew Coveyou
Curation notes

Experimental Process

The promoter region of Pseudomonas putida PRS2000 gene catR was determined by reverse transcriptase mapping. Beta-gal assay of both the catR gene promoter and catBCA operon promoter indicated constitutive autoregulation of catR and inducer-dependent activation of catBCA. CatR was purified via DNA affinity column, and functionality was confirmed by EMSA. Hydroxyl-radical footprinting identified three points of direct contact between the promoter and catR across 24 bp. Footprints were not influence by the presence of the inducer.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... catB catC catA catR PP_3712
Experimental technique details Beta-gal reporter assay - Experimental technique details DNA affinity purification (ECO:0005629) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Hydroxyl-radical footprinting (ECO:0005643) - activator not specified