Visual inspection of the cupD upstream region identified a putative RcsB binding site. RACE PCR determined the transcriptional start site location of cupD. qRT-PCR demonstrated RcsB-mediated activation of cupD transcription. These results were confirmed with cupD'-lacZ reporter assays. Multiple sequence alignment of RcsB-binding regions and putative binding sites further characterized the RcsB binding motif.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|TAAGAAACGTCCTAAA||cupD1, cupD2, cupD3, cupD4, cupD5||