EMSA with a 352-bp todX promoter fragment showed that up to four different complexes were formed. A series of ITC assays further verified binding of TodT to todX promoter region. Beta-galactosidase assays showed that mutagenesis of both half-palindromes resulted in an almost 12-fold decrease in transcriptional activity.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|