Transcription start sites of divergently transcribed trpBA and trpI were identified by Sl nuclease mapping. DNase I footprinting was used to locate the TrpI binding site. TrpI binding site was further characterized by hydroxyl radical footprinting. Alignment of the trpBA-trpI intergenic regions from P.aeruginosa PAO1 and P.putida PPGI identified the putative consensus sequence of the TrpI binding site.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
||not specified||not specified|