Curation Information

Publication

Cross-regulation of biphenyl- and salicylate-catabolic genes by two regulatory systems in Pseudomonas pseudoalcaligenes KF707.;Fujihara H, Yoshida H, Matsunaga T, Goto M, Furukawa K;Journal of bacteriology 2006 Jul; 188(13):4690-7 [16788178]

TF

BphR2 [I3XE46, view regulon]

Reported TF sp.

Pseudomonas pseudoalcaligenes KF707

Reported site sp.

Pseudomonas pseudoalcaligenes KF707

Created by

Grace Chandler

Curation notes

There is no appropriate ref_seq genome for this strain of Pseudomonas. The TF accession numbers used were from Sinorhizobium fredii USDA 257.

Experimental Process

qRT-PCR demonstrated positive regulation of sal genes by BphR2. EMSA confirmed binding of BphR2 to the salA promoter. DNase I footprinting localized the BphR2 binding sites in the salA promoter region, both on the sense and antisense strands.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.