Curation Information

Cross-regulation of biphenyl- and salicylate-catabolic genes by two regulatory systems in Pseudomonas pseudoalcaligenes KF707.;Fujihara H, Yoshida H, Matsunaga T, Goto M, Furukawa K;Journal of bacteriology 2006 Jul; 188(13):4690-7 [16788178]
BphR2 [I3XE46, view regulon]
Reported TF sp.
Pseudomonas pseudoalcaligenes KF707
Reported site sp.
Pseudomonas pseudoalcaligenes KF707
Created by
Grace Chandler
Curation notes
There is no appropriate ref_seq genome for this strain of Pseudomonas. The TF accession numbers used were from Sinorhizobium fredii USDA 257.

Experimental Process

qRT-PCR demonstrated positive regulation of sal genes by BphR2. EMSA confirmed binding of BphR2 to the salA promoter. DNase I footprinting localized the BphR2 binding sites in the salA promoter region, both on the sense and antisense strands.

Transcription Factor Binding Sites

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type