EMSA demonstrated binding of IHF to the Pu promoter. DNase I footprinting identified the location of the IHF binding site. Site directed mutagenesis combined with EMSA confirmed the identity and specificity of this promoter sequence.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|