TtgV binding to a 42-bp region in the ttgV-ttgG intergenic region had been shown previously by DNase I footprinting and DMS footprinting assays. Isothermal titration calorimetry experiments revealed that a 34 bp region containing two inverted repeats corresponds to a functional operator recognized by TtgV. Removal of the left half-site of IR1 or alteration of the right half-site of IR2 prevented binding of TtgV to the target operator. Alteration of the left half-site of IR2 or removal of the right half-site of IR1 resulted in low albeit measurable binding of TtgV to its operator.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|