ttgG-lacZ and ttgV-lacZ reporter assays in the wild-type DOT-T1E and in the ttgV mutant strains identified that TtgV represses the expression of both ttgVW and ttgGHI operons. EMSA showed that TtgV bound to a 210-bp DNA fragment containing the sequence of the ttgV-ttgG intergenic region. Footprinting assays defined the region TtgV binding region within the ttgV-ttgG intergenic region.
Quantitative data format: Kd
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|