Curation Information

The non-classical ArsR-family repressor PyeR (PA4354) modulates biofilm formation in Pseudomonas aeruginosa.;Mac Aogáin M, Mooij MJ, McCarthy RR, Plower E, Wang YP, Tian ZX, Dobson A, Morrissey J, Adams C, O'Gara F;Microbiology (Reading, England) 2012 Oct; 158(Pt 10):2598-609 [22820840]
PyeR [Q9HW47, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Grace Chandler
Curation notes

Experimental Process

Beta galactosidase assays using a pyeR deletion mutant and the wild-type PAO1 strains identified that PyeR acts as an auto-repressor. Motif discovery using MEME determined a PyeR binding motif in the upstream regions of the pyeR orthologs. EMSA demonstrated that PyeR binds directly to its own promoter, consistent with PyeR's role as an auto-regulator. qRT-PCR identified that pyeR, pyeM and xenB are all co-transcribed in one operon.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... PA4354 PA4355 xenB PA4353 PA4352
Experimental technique details Beta-gal reporter assay - Experimental technique details EMSA (ECO:0001807) - Experimental technique details Motif-discovery (ECO:0005558) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - repressor not specified