Beta galactosidase assays using a pyeR deletion mutant and the wild-type PAO1 strains identified that PyeR acts as an auto-repressor. Motif discovery using MEME determined a PyeR binding motif in the upstream regions of the pyeR orthologs. EMSA demonstrated that PyeR binds directly to its own promoter, consistent with PyeR's role as an auto-regulator. qRT-PCR identified that pyeR, pyeM and xenB are all co-transcribed in one operon.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|ATATCGTCCCACACCGATATATAGTTCGTCCCAACCCGAT||PA4354, PA4355, xenB,||