Curation Information

The Pseudomonas aeruginosa Vfr regulator controls global virulence factor expression through cyclic AMP-dependent and -independent mechanisms.;Fuchs EL, Brutinel ED, Jones AK, Fulcher NB, Urbanowski ML, Yahr TL, Wolfgang MC;Journal of bacteriology 2010 Jul; 192(14):3553-64 [20494996]
Vfr [P55222, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAK
Reported site sp.
Pseudomonas aeruginosa PAK
Created by
Grace Chandler
Curation notes

Experimental Process

EMSA using the vfr promoter identified specific Vfr binding. In vitro transcription assays determined Vfr and cyclic AMP are required to activate vfr. DNase I footprinting localized the Vfr promoter region which contained the binding site. Beta-gal assays verified this cAMP dependence and Vfr mediated regulation.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... vfr PA0653
Experimental technique details Beta-gal reporter assay - Experimental technique details DNAse footprinting (ECO:0005631) - Experimental technique details EMSA (ECO:0001807) - Experimental technique details In-vitro transcription (ECO:0001204) - activator not specified