LecA transcription start site was determined by primer extension and S1 nuclease analysis. lecA-lux reporter assay results suggested that lecA expression is directly regulated by RhlR. A putative RhlR binding site was verified by multiple sequence alignment with luxI, rhlA, and rhlI and the lasB-OP1 genes.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|