Curation Information

Publication
The Pseudomonas aeruginosa lectins PA-IL and PA-IIL are controlled by quorum sensing and by RpoS.;Winzer K, Falconer C, Garber NC, Diggle SP, Camara M, Williams P;Journal of bacteriology 2000 Nov; 182(22):6401-11 [11053384]
TF
LasR [P54292, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

LecA transcription start site was determined by primer extension and S1 nuclease analysis. lecA-lux reporter assay results suggested that lecA expression is directly regulated by RhlR. A putative RhlR binding site was verified by multiple sequence alignment with luxI, rhlA, and rhlI and the lasB-OP1 genes.

Transcription Factor Binding Sites


TCCTGCATGAATTGGTAGGC
TCCTGCATGAATTGGTAGGC

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TCCTGCATGAATTGGTAGGC lecA
... ... lecA
Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - activator not specified