2D gel electrophoresis was used to compare the protein patterns of the P. aeruginosa narL mutant with that of the wild-type PAO1. Northern blot analysis in wild type and narL mutant strains showed that NarL represses arcDABC expression. LacZ reporter assays in the wild type and narL mutant confirmed arcDABC repression by NarL. Site-directed mutagenesis of the putative NarL binding site in conjunction with arcD-lacZ fusion assays confirmed direct repression of arcDABC by NarL.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|TACTCAA||arcD, arcA, arcB, arcC||