Curation Information

Publication
Nitrate-responsive NarX-NarL represses arginine-mediated induction of the Pseudomonas aeruginosa arginine fermentation arcDABC operon.;Benkert B, Qu├Ąck N, Schreiber K, Jaensch L, Jahn D, Schobert M;Microbiology (Reading, England) 2008 Oct; 154(Pt 10):3053-60 [18832311]
TF
NarL [Q9HXD3, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

2D gel electrophoresis was used to compare the protein patterns of the P. aeruginosa narL mutant with that of the wild-type PAO1. Northern blot analysis in wild type and narL mutant strains showed that NarL represses arcDABC expression. LacZ reporter assays in the wild type and narL mutant confirmed arcDABC repression by NarL. Site-directed mutagenesis of the putative NarL binding site in conjunction with arcD-lacZ fusion assays confirmed direct repression of arcDABC by NarL.

Transcription Factor Binding Sites


TACTCAA
TACTCAA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TACTCAA arcD, arcA, arcB, arcC
... ... arcD arcA arcB arcC
Experimental technique details 2D PAGE (ECO:0005614) - Experimental technique details Beta-gal reporter assay - Experimental technique details Northern blot (ECO:0005653) - Experimental technique details Site directed mutagenesis (ECO:0005667) - repressor not specified