Curation Information

The Pseudomonas aeruginosa rmlBDAC operon, encoding dTDP-L-rhamnose biosynthetic enzymes, is regulated by the quorum-sensing transcriptional regulator RhlR and the alternative sigma factor σS.;Aguirre-Ramírez M, Medina G, González-Valdez A, Grosso-Becerra V, Soberón-Chávez G;Microbiology (Reading, England) 2012 Apr; 158(Pt 4):908-16 [22262098]
LasR [P54292, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Dinara Sagitova
Curation notes

Experimental Process

rmlBDAC operon transcription start was determined by primer extension analysis. A putative RhlR binding site resembling a las box was identified by visual inspection of the rmlBDAC promoter. rmlBDAC-lacZ fusion assays using rhlR mutant strain showed the expression of the rmlBDAC operon in early stationary phase is dependent on RhlR. Deletion of the putative binding site resulted in lower expression levels in lacZ reporter assays. Site-directed mutagenesis of the RhlR binding site completely abolished the induction of the lacZ promoter fusion by RhlR.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... rmlB rmlD rmlA rmlC PA5165 PA5166
Experimental technique details Beta-gal reporter assay - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator not specified