Crp-mediated activation of phhAB operon was confirmed by phhAB-lacZ fusion assays in the wild-type and Crp and Cya mutant strains. EMSA revealed that the Crp bound to the phhAB promoter when cAMP was present. DNase I footprinting identified the Crp binding region in the phhAB promoter. Random mutagenesis of the putative Crp binding site confirmed that Crp binding to its target site is specific.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|