Curation Information

GacA-controlled activation of promoters for small RNA genes in Pseudomonas fluorescens.;Humair B, Wackwitz B, Haas D;Applied and environmental microbiology 2010 Mar; 76(5):1497-506 [20048056]
PsrA [Q4KFB4, view regulon]
Reported TF sp.
Pseudomonas fluorescens CHA0
Reported site sp.
Pseudomonas fluorescens CHA0
Created by
Dinara Sagitova
Curation notes

Experimental Process

Multiple sequence alignment of rsmX, rsmY, and rsmZ promoters revealed a conserved palindromic UAS. The rsmZ-lacZ reporter assays showed that deletion of the UAS in rsmZ promoter resulted in almost complete loss of transcription in strain CHA0 growing in NYB. The rsmZ-lacZ fusion showed reduced and delayed expression in the psrA deletion mutant compared to that in wild-type strain. A potential PsrA recognition sequence was found overlapping with the UAS in the rsmZ promoter region. When this sequence was mutated either by a 3-bp deletion or by a 3-bp change, neither of which altered the UAS, regulation by PsrA appeared to be lost.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... rsmZ
Experimental technique details Beta-gal reporter assay - Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) - Experimental technique details Site directed mutagenesis (ECO:0005667) - Experimental technique details Visual sequence inspection (nan) - activator not specified