Multiple sequence alignment of rsmX, rsmY, and rsmZ promoters revealed a conserved palindromic UAS. The rsmZ-lacZ reporter assays showed that deletion of the UAS in rsmZ promoter resulted in almost complete loss of transcription in strain CHA0 growing in NYB. The rsmZ-lacZ fusion showed reduced and delayed expression in the psrA deletion mutant compared to that in wild-type strain. A potential PsrA recognition sequence was found overlapping with the UAS in the rsmZ promoter region. When this sequence was mutated either by a 3-bp deletion or by a 3-bp change, neither of which altered the UAS, regulation by PsrA appeared to be lost.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|