An aotJ::lacZ fusion showed the aot operon is strongly induced by arginine only when ArgR is present. EMSA was used with ArgR to demonstrate binding to the aotJ regulatory region. DNase I footprinting, premethylation and depurination showed a 44 bp protected region. Multiple sequence alignment produced an ArgR consensus binding sequence.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|CTTCGGTGCTTTTATAATTAGTTGTCGCATTGAAGAAATAACC||aotJ, aotQ, aotM, PA0891, aotP, argR,||