Curation Information

The homogentisate pathway: a central catabolic pathway involved in the degradation of L-phenylalanine, L-tyrosine, and 3-hydroxyphenylacetate in Pseudomonas putida.;Arias-Barrau E, Olivera ER, Luengo JM, Fernández C, Galán B, García JL, Díaz E, Miñambres B;Journal of bacteriology 2004 Aug; 186(15):5062-77 [15262943]
HmgR [Q88E47, view regulon]
Reported TF sp.
Pseudomonas putida KT2440
Reported site sp.
Pseudomonas putida KT2440
Created by
Joe Sparenberg
Curation notes

Experimental Process

In vivo transcriptional analyses showed that HmgR is a repressor of the hmgABC operon. lacZ promoter fusion analysis further confirmed the results. EMSA confirmed HmgR binding to hmgR-hmgA intergenic region. DNase I footprinting was used to localize the HmgR binding region.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type