Curation Information

Publication
Conservation of the Pho regulon in Pseudomonas fluorescens Pf0-1.;Monds RD, Newell PD, Schwartzman JA, O'Toole GA;Applied and environmental microbiology 2006 Mar; 72(3):1910-24 [16517638]
TF
PhoB [Q3K4G1, view regulon]
Reported TF sp.
Pseudomonas fluorescens Pf0-1
Reported site sp.
Pseudomonas fluorescens Pf0-1
Created by
Dinara Sagitova
Curation notes
-

Experimental Process

Pho boxes similar to the E.coli consensus were identified in the pst and phoD genes. RT-PCR analysis of the WT and pst mutant strains grown under Pi-sufficient conditions showed that phoX and phoD expression was upregulated in the pst mutant strain. Luciferase reporter assays with phoD promoters containing the original and a mutated pho box were performed in WT and phoB mutant strains. The results of these assays confirmed PhoB-dependent activation of phoD.

Transcription Factor Binding Sites


CTTTCATCCAATTGTCA
TTGTCATATTTCAGCCA
CTGCAACGAAAGCGTCA
GTGTCACACCCCTTTCA
CTTTCATCCAATTGTCA
TTGTCATATTTCAGCCA
CTGCAACGAAAGCGTCA
GTGTCACACCCCTTTCA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
CTTTCATCCAATTGTCA Pfl01_5615
... ... Pfl01_5615
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified
TTGTCATATTTCAGCCA Pfl01_5615
... ... Pfl01_5615
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified
CTGCAACGAAAGCGTCA Pfl01_5179,
... ... Pfl01_5179 Pfl01_5180 Pfl01_5181
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - activator not specified
GTGTCACACCCCTTTCA Pfl01_0796
... ... Pfl01_0796
Experimental technique details Consensus search (ECO:0005624) - Experimental technique details Luciferase reporter assay (ECO:0005648) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Site directed mutagenesis (ECO:0005667) - activator not specified