Pho boxes similar to the E.coli consensus were identified in the pst and phoD genes. RT-PCR analysis of the WT and pst mutant strains grown under Pi-sufficient conditions showed that phoX and phoD expression was upregulated in the pst mutant strain. Luciferase reporter assays with phoD promoters containing the original and a mutated pho box were performed in WT and phoB mutant strains. The results of these assays confirmed PhoB-dependent activation of phoD.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|