Curation Information

Purification and characterization of CopR, a transcriptional activator protein that binds to a conserved domain (cop box) in copper-inducible promoters of Pseudomonas syringae.;Mills SD, Lim CK, Cooksey DA;Molecular & general genetics : MGG 1994 Aug 15; 244(4):341-51 [8078459]
CopR [Q87Z28, view regulon]
Reported TF sp.
Pseudomonas syringae PT23.2
Reported site sp.
Pseudomonas syringae PT23.2
Created by
Grace Chandler
Curation notes

Experimental Process

Site-directed mutagenesis combined with lacZ transcriptional fusion narrowed down the region of cop promoter necessary for copper inducible expression. EMSA determined that CopR binds specifically to the cop promoter. DNase I footprinting localized the CopR binding site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type