Curation Information

Characterization of interactions between the transcriptional repressor PhlF and its binding site at the phlA promoter in Pseudomonas fluorescens F113.;Abbas A, Morrissey JP, Marquez PC, Sheehan MM, Delany IR, O'Gara F;Journal of bacteriology 2002 Jun; 184(11):3008-16 [12003942]
PhlF [G8QCB1, view regulon]
Reported TF sp.
Pseudomonas fluorescens F113
Reported site sp.
Pseudomonas fluorescens F113
Created by
Grace Chandler
Curation notes

Experimental Process

phlF-lacZ and phlA-lacZ fusion assays determined phlABCD repression by PhlF in the early logarithmic growth phase of P. fluorescens, with PHL inhibiting this repression in later growth stages. EMSA using DNA fragments of varied lengths confirmed PhlF binding to the phlA-phlF intergenic region. Copper-phenanthroline footprinting localized the binding site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type