EMSAs were performed on the 94-bp mexL-mexJ intergenic region, demonstrating that MexL binds specifically. Visual inspection revealed two inverted GTATTT repeats on the mexL-mexJ intergenic region, separated by 16 bp. DNase I footprinting was performed to further map the operator sites, revealing a single protected region on both the mexL and mexJ coding strands, with the regions almost perfectly overlapping. LacZ fusion assays demonstrated auto-repression of mexL with expression levels lower in the presence of MexL.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|