EMSA was performed on a deletion series of the aat promoter, identified that AauR binds to the aat promoter region in the -117 to -136 range. A DNAse footprinting assay using an aat promoter fragment from -83 to -246 demonstrated that a 19 bp section was protected. Alignment of the aat promoter with homogolous genes from other Pseudomonas spp. revealed that the putative AauR binding motif (TTCGGNNNNCCGAA) is well conserved in other pseudomonads. In a separate study, AauR-dependent activation of aatJMQP was shown by Sonawane et al., 2006.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|CGTTCGGATAACCGAACGA||PP_1071, PP_1070, PP_1069, PP_1068,||