Transcription start sites for gltBD and gdhA promoters were identified by primer extension analysis. gltB::lacZ and gdhA:: lacZ fusion assays showed repression by ArgR in the presence of arginine in the wild type but not in the argR knockout mutant. EMSA demonstrated that ArgR specifically binds to the gltBD regulatory region. Similarly, EMSAs showed that ArgR binds to gdhA promoter. DNAse I footprinting identified the location of the binding sites in the gdhA and gltBD promoter regions. Multiple sequence alignment determined an ArgR consensus binding motif.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|