QRT-PCR analysis was performed with an arnR deficient strain and compared to a wild type strain to identify genes regulated by arnR. The results show that ArnR represses the narKGHI operon and the hmp gene. Results were confirmed with DNA microarray analyses. LacZ reporter assay shows that arnR is positively autoregulated by its own gene product. EMSAs were performed and results show that ArnR directly regulates transcription of narKGHI operon, hmp gene and arnR. EMSAs were also performed using different size fragments to pinpoint the location of the promoter region. TAWTTAAWTA was inferred as a consensus sequence determined by primer extension analysis. These results were confirmed with DNase I footprinting analysis.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|TAATTAAATA||cgR_1269, cgR_1268, cgR_1267, cgR_1266, cgR_1265,||