Curation Information

Phosphate limitation induces catalase expression in Sinorhizobium meliloti, Pseudomonas aeruginosa and Agrobacterium tumefaciens.;Yuan ZC, Zaheer R, Finan TM;Molecular microbiology 2005 Nov; 58(3):877-94 [16238634]
PhoB [P23620, view regulon]
Reported TF sp.
Pseudomonas aeruginosa PAO1
Reported site sp.
Pseudomonas aeruginosa PAO1
Created by
Matthew Coveyou
Curation notes

Experimental Process

A consensus search identified the putative binding site. GUS assays of PhoB variable strains grown in phosphate-limited media demonstrated strong expression of the binding site in the PhoB+ strain but not the PhoB- strain. EMSA identified strong binding of PhoB to the putative site.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
... ... katA bfrA
Experimental technique details EMSA (ECO:0001807) - Experimental technique details GUS reporter gene assay (ECO:0005641) - Experimental technique details PSSM site search (ECO:0005659) - activator not specified