Curation Information

Publication

The Pseudomonas aeruginosa global regulator MvaT specifically binds to the ptxS upstream region and enhances ptxS expression.;Westfall LW, Luna AM, San Francisco M, Diggle SP, Worrall KE, Williams P, Cámara M, Hamood AN;Microbiology (Reading, England) 2004 Nov; 150(Pt 11):3797-806 [15528665]

TF

MvaT [Q9HW86, view regulon]

Reported TF sp.

Pseudomonas aeruginosa PAO1

Reported site sp.

Pseudomonas aeruginosa PAO1

Created by

Grace Chandler

Curation notes

-

Experimental Process

EMSA was performed on DNA fragments of varying lengths in order to localize the binding activity of MvaT. DNAse I footprinting identified the MvaT binding sequence. RT-PCR demonstrated the effect of MvaT as an activator of ptxS expression.

Transcription Factor Binding Sites

• Sites reported in the paper
• Sites matched in the genome

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.