qRT-PCR was used to determine that the irr gene expression is regulated by both iron and manganese. DNAse I footprinting identified the Irr binding site within the -64 to -39 region of the Irr promoter.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|